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Glycosphingolipid 항원의 대량 Spectrometric 분석
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Mass Spectrometric Analysis of Glycosphingolipid Antigens
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Glycosphingolipid 항원의 대량 Spectrometric 분석

16,291 Views

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13:09 min

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April 16, 2013

DOI:

10.3791/4224-v

DOI:
10.3791/4224-v

•

13:09 min
April 16, 2013

•

9 Views
, ,
1Undergraduate Program,Rice University , 2Proteomics Facility, Department of Pathology,University of Texas MD Anderson Cancer Center , 3Department of Melanoma Medical Oncology,University of Texas MD Anderson Cancer Center , 4University of Texas Graduate School of Biological Sciences at Houston

Summary

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면역 기관과 세포의 glycosphingolipid 항원의 표현 프로필에 대한 통찰력을 얻을 수있는 구체적이고 민감한 방법을 설명합니다. 방법은 화학적으로 합성 표준에 비해 구조 분석을위한 glycosphingolipid 분자의 단계 - 현명한 조각을 허용 이온 트랩 질량 분광법을 활용합니다.

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Cite this Article

Yin, A. B., Hawke, D., Zhou, D. Mass Spectrometric Analysis of Glycosphingolipid Antigens. J. Vis. Exp. (74), e4224, doi:10.3791/4224 (2013).

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