Journal
/
Biología
/
在体内电穿孔Morpholinos到成年斑马鱼视网膜
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
Please note that all translations are automatically generated. Click here for the English version.

在体内电穿孔Morpholinos到成年斑马鱼视网膜

DOI:
10.3791/3603-v

•

06:55 min

•

December 27, 2011

•

, ,
1Departments of Anatomy and Cell Biology and Ophthalmology,Wayne State University School of Medicine, 2Department of Biological Sciences,University of Notre Dame , 3Center for Zebrafish Research,University of Notre Dame

Capítulos

  • 00:05Título
  • 01:28Morpholino Preparation and Removing the Outer Corneal Layer
  • 02:32Corneal Incision and Morpholino Injection
  • 03:31Electroporation
  • 04:55Representative Morpholino Electroporation Results
  • 06:14Conclusion

Summary

Traducción Automática

Traducción Automática

有条件击倒在成年斑马鱼视网膜的目标蛋白质的表达，是一个方法，它涉及到玻璃体内注射反义morpholinos和电穿孔进入视网膜。由此产生的蛋白质是撞倒了好几天​​，这使得检测蛋白质的作用，在再生或完整的视网膜。

Tags

In Vivo ElectroporationMorpholinosAdult Zebrafish RetinaInherited Eye DiseasesProgressive BlindnessRetinal NeuronsRegenerationRetinal Damage ModelsPhotoreceptorsInner Retinal NeuronsMüller GliaNeuronal ProgenitorsRetinal LayerDifferentiationCellular ResponsesMRNA Microarray AnalysisOcular DevelopmentCandidate Gene/protein

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

在成年斑马鱼的全肾骨髓移植

器官型培养的成年兔视网膜

在体内的单细胞电穿孔内完整的大脑发育

在成年斑马鱼的眼窝射出

剖析器官的成年斑马鱼

腹腔注射到成年斑马鱼

通过注射和电穿孔的斑马鱼Otocyst的MIF Morpholinos直接运抵影响内耳发展

直接进入成年斑马鱼的肾细胞移植

成年斑马鱼肾脏的解剖

再生的成年斑马鱼尾鳍在吗啉代入体内电穿孔

Read Article