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In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
Chapters
- 00:05Title
- 01:28Morpholino Preparation and Removing the Outer Corneal Layer
- 02:32Corneal Incision and Morpholino Injection
- 03:31Electroporation
- 04:55Representative Morpholino Electroporation Results
- 06:14Conclusion
A method to conditionally knockdown a target protein’s expression in the adult zebrafish retina is described, which involves intravitreally injecting antisense morpholinos and electroporating them into the retina. The resulting protein is knocked down for several days, which allows testing the protein’s role in the regenerating or intact retina.
Tags
In Vivo ElectroporationMorpholinosAdult Zebrafish RetinaInherited Eye DiseasesProgressive BlindnessRetinal NeuronsRegenerationRetinal Damage ModelsPhotoreceptorsInner Retinal NeuronsMüller GliaNeuronal ProgenitorsRetinal LayerDifferentiationCellular ResponsesMRNA Microarray AnalysisOcular DevelopmentCandidate Gene/protein
