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In vivo Elektroporatie van Morpholinos in de Adult zebravis Retina
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Biología
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JoVE Revista Biología
In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
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In vivo Elektroporatie van Morpholinos in de Adult zebravis Retina

18,774 Views

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06:55 min

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December 27, 2011

DOI:

10.3791/3603-v

DOI:
10.3791/3603-v

•

06:55 min
December 27, 2011

•

5 Views
, ,
1Departments of Anatomy and Cell Biology and Ophthalmology,Wayne State University School of Medicine, 2Department of Biological Sciences,University of Notre Dame , 3Center for Zebrafish Research,University of Notre Dame

Summary

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Een methode om een ​​doelwit eiwit expressie voorwaardelijk knockdown in de volwassen zebravissen netvlies wordt beschreven, wat inhoudt intravitreally injecteren van antisense morpholinos en electroporating ze in het netvlies. De daaruit resulterende eiwitten neergeslagen voor meerdere dagen, waardoor het testen van de eiwit rol in de regenereren of intact netvlies.

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Cite this Article

Thummel, R., Bailey, T. J., Hyde, D. R. In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina. J. Vis. Exp. (58), e3603, doi:10.3791/3603 (2011).

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