Journal
/
Neurociencias
/
Cultura e Eletrofisiologia de células em NRCC patch-clamp-Chips
JoVE Revista
Neurociencias
This content is Free Access.
JoVE Revista Neurociencias
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Cultura e Eletrofisiologia de células em NRCC patch-clamp-Chips

15,426 Views

•

10:36 min

•

February 07, 2012

DOI:

10.3791/3288-v

DOI:
10.3791/3288-v

•

10:36 min
February 07, 2012

•

8 Views
, , , , , , ,
1Institute for Microstructural Sciences,National Research Council of Canada, 2Institute for Biological Sciences,National Research Council of Canada, 3Hotchkiss Brain Institute,University of Calgary

Summary

Automatically generated

Nós mostramos como planar de patch-clamp fichas fabricado no National Research Council of Canada são esterilizados, preparado, carregado com médio, revestida com as células, e usado para gravações eletrofisiológicas.

Explore More Videos

CulturingElectrophysiologyCellsPatch-clampChipsSensitivityIon ChannelsGold StandardDisease ModelsPharmaceutical ScreensGlass PipetteMembrane PatchElectrodeIon-channel ActivityWhole CellPatch-clamp ChipsSuspended FilmAperture MicrofabricatedAutomated SystemsHigh-throughput ScreeningFluidic DeliverySuctionCell-to-probe SealsWhole Cell ModeSilicon Patch-clamp ChipImpedanceOrifice ShapeAction PotentialsSnail NeuronsMammalian Neurons

Read Article

Cite this Article

Py, C., Martina, M., Monette, R., Comas, T., Denhoff, M. W., Luk, C., Syed, N. I., Mealing, G. Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips. J. Vis. Exp. (60), e3288, doi:10.3791/3288 (2012).

Download .ris file

Copy

Share Video

.

Copy