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Lectin-basierte Isolierung und Kultivierung von embryonalen Maus-Motoneuronen
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons
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Lectin-basierte Isolierung und Kultivierung von embryonalen Maus-Motoneuronen

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09:25 min

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September 15, 2011

DOI:

10.3791/3200-v

DOI:
10.3791/3200-v

•

09:25 min
September 15, 2011

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, , , , ,
1Institute for Cellmorphology and molecular Neurobiology, Group for Cellbiology,Ruhr-University Bochum, 2Institute for Clinical Neurobiology,University of Wuerzburg

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Lectin-based IsolationCultureMouse Embryonic MotoneuronsSpinal MotoneuronsNeuroepithelial CellsNkx6.1Precursor CellsPostmitotic StagesDendritesAxonsTranscription FactorsIslet 1/2Innervate MusclesHigh Affinity ReceptorsBrain Derived Neurotrophic Factor (BDNF)Neurotrophin-3 (NT-3)Tropomyosin-related Kinase B And C (TrkBTrkC)Tropomyosin-related Kinase A (TrkA)Low Affinity Neurotrophin Receptor P75NTRIsolation TechniquesSpecific Antibodies (panning)

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Conrad, R., Jablonka, S., Sczepan, T., Sendtner, M., Wiese, S., Klausmeyer, A. Lectin-based Isolation and Culture of Mouse Embryonic Motoneurons. J. Vis. Exp. (55), e3200, doi:10.3791/3200 (2011).

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