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12:45 min
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August 16, 2011
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Summary
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我们描述了从被感染的细胞中分离高纯度的疱疹病毒衣壳DNA的过程。从溶液中捕获的是最后的DNA浓度和纯度高，使得它非常适合高通量测序，高保真PCR反应，并产生新的病毒重组体转染。
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Viral DNANucleocapsidsHost Cell ContaminantsDownstream ApplicationsViral Genome SequencingHost DNA RemovalViral Recombinant StrainsPurified PlasmidLinear Viral DNAExtractionsDensity-based CentrifugationPurified Viral CapsidsCellular ProteinsOD260/280 RatioYields Of Viral DNALong-term StorageHigh-throughput SequencingHigh Fidelity PCR ReactionsTransfections
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Cite this Article
Szpara, M. L., Tafuri, Y. R., Enquist, L. W. Preparation of Viral DNA from Nucleocapsids. J. Vis. Exp. (54), e3151, doi:10.3791/3151 (2011).
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