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Capítulos
- 00:05Título
- 02:08Tosylactivated Bead Coating
- 04:25PBMC Isolation
- 05:41MACS Pre-sort of CD4 T Cells
- 08:05Fluorescent Activated Cell Sorting (FACS) Isolation
- 09:59Cell Culture Setup, Harvesting, and Counting
- 11:32Tregs Differ in their Ability to Successfully Suppress Naive and in vivo-activated T Cells
- 13:34Conclusion
Tags
Human In Vitro SuppressionScreening ToolRecognitionEarly StateImmune ImbalanceRegulatory T CellsTregsImmune ToleranceSelf-antigensInfectionSurface MarkerProliferationFunctionEffector T CellsIn Vitro AssaysCross-sectional StudiesAutoimmune DiseasesType 1 Diabetes-T1DLaboratoriesResponder T CellsStimulationTreg:responder RatiosAntigen-presenting Cells (APC)VariabilityComparison Between StudiesStandardized Suppression AssayHealthy SubjectsAutoimmune Destruction Of Pancreatic β-cells
