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人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具
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Inmunología y contagio
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Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance
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人类<em在体外承认免疫失衡的早期国家禁止作为筛检工具

DOI:
10.3791/3071-v

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14:01 min

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July 22, 2011

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, ,
1Department of Pediatrics/Allergy,Medical College of Wisconsin , 2Flow Cytometry Core Facility,Medical College of Wisconsin , 3Max McGee National Research Center for Juvenile Diabetes and Human Molecular Genetics Center,Medical College of Wisconsin

Capítulos

  • 00:05Título
  • 02:08Tosylactivated Bead Coating
  • 04:25PBMC Isolation
  • 05:41MACS Pre-sort of CD4 T Cells
  • 08:05Fluorescent Activated Cell Sorting (FACS) Isolation
  • 09:59Cell Culture Setup, Harvesting, and Counting
  • 11:32Tregs Differ in their Ability to Successfully Suppress Naive and in vivo-activated T Cells
  • 13:34Conclusion

Summary

Traducción Automática

Traducción Automática

调节性T细胞是免疫系统的强有力的抑制器。有一个独特的表面标志物来定义它们的缺乏，因此，调节性T细胞的定义主要功能。在这里，我们描述了一个优化的<em>在体外</em>检测能够识别风险科目的免疫失衡发展T1D。

Tags

Human In Vitro SuppressionScreening ToolRecognitionEarly StateImmune ImbalanceRegulatory T CellsTregsImmune ToleranceSelf-antigensInfectionSurface MarkerProliferationFunctionEffector T CellsIn Vitro AssaysCross-sectional StudiesAutoimmune DiseasesType 1 Diabetes-T1DLaboratoriesResponder T CellsStimulationTreg:responder RatiosAntigen-presenting Cells (APC)VariabilityComparison Between StudiesStandardized Suppression AssayHealthy SubjectsAutoimmune Destruction Of Pancreatic β-cells

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