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从部分Pancreatectomized患者的人类胰岛的分离

JoVE Revista
Medicina
Author Produced

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JoVE Revista Medicina

Isolation of Human Islets from Partially Pancreatectomized Patients

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从部分Pancreatectomized患者的人类胰岛的分离

DOI:

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11:10 min

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July 30, 2011

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Gregor Bötticher, Dorothèe Sturm, Florian Ehehalt, Klaus P. Knoch, Stephan Kersting, Robert Grützmann, Gustavo B. Baretton, Michele Solimena, Hans D. Saeger

¹Department of GI-, Thorax- and Vascular Surgery,University Hospital Carl Gustav Carus, University of Technology Dresden, ²Molecular Diabetology,Paul Langerhans Institute Dresden, ³Department of Pathology,University Hospital Carl Gustav Carus, University of Technology Dresden

Capítulos

00:03Título
00:05Introduction
00:54Surgical Procedure
01:35Pathology
07:01Collection and Culture
09:07Conclusion

Summary

Traducción Automática

为研究2型糖尿病胰岛供应不足。在这里，我们分享我们的协议隔离部分胰腺切除术病人的胰岛。这种方法代表一个独特的场地和2型糖尿病胰岛获得足够数量的非糖尿病患者的临床基础和临床研究相匹配。

Tags

Human Islets Isolation Partially Pancreatectomized Patients Type 2 Diabetes Islets Of Langerhans Malfunction Limited Availability Organ Donors Partial Pancreatectomy Pancreatic Diseases Consent Local Ethics Committee Surgical Specimens Diagnostic Purposes 1 000 Islets Enzyme-containing Media Digestion Surface Area Ricordi Chamber

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