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Fluorescence Lifetime Imaging für molekulare Rotoren in lebenden Zellen
JoVE Revista
Bioingeniería
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JoVE Revista Bioingeniería
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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Fluorescence Lifetime Imaging für molekulare Rotoren in lebenden Zellen

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09:45 min

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February 09, 2012

DOI:

10.3791/2925-v

DOI:
10.3791/2925-v

•

09:45 min
February 09, 2012

•

4 Views
, , , ,
1Department of Physics,King’s College London, 2Department of Chemistry,Imperial College London , 3PhotoBiotics Ltd

Summary

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Fluorescence Lifetime Imaging (FLIM) wurden als wesentliche Technik, um Bild die Umwelt und die Interaktion bestimmter Proteine ​​und Farbstoffe, die in lebenden Zellen entstanden. FLIM fluoreszierender molekularer Rotoren ermöglicht die Zuordnung der Viskosität in lebenden Zellen.

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Suhling, K., Levitt, J. A., Chung, P. H., Kuimova, M. K., Yahioglu, G. Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells. J. Vis. Exp. (60), e2925, doi:10.3791/2925 (2012).

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