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Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury
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Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury
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July 20, 2011
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We present a method for chromatin immunoprecipitation from dorsal root ganglia tissue following axonal injury. The approach can be used to identify specific transcription factor binding sites and epigenetic modification of histone and DNA important for the regeneration of injured axons in both the peripheral and central nervous system.
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Chromatin ImmunoprecipitationDorsal Root GangliaAxonal InjuryCentral Nervous SystemPeripheral Nervous SystemNeurite OutgrowthAxonal RegenerationTranscriptional ProgramsGene RegulationInjury Model SystemTranscriptional ActivityAnimal RemovalNerve FibersSciatic NerveSpinal CordTranscription Factor BindingHistone ModificationDNA ModificationChIP Protocol
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Floriddia, E., Nguyen, T., Di Giovanni, S. Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury. J. Vis. Exp. (53), e2803, doi:10.3791/2803 (2011).
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