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Chromatin Immunoprecipitation from Dorsal Root Ganglia Tissue following Axonal Injury
Chapters
- 00:05Title
- 01:04Sciatic & Dorsal Column Nerve Injury
- 04:35Cross-linking
- 06:14Immunoprecipitation and Elution
- 08:23Increased Expression After Nerve Injury
- 09:11Conclusion
We present a method for chromatin immunoprecipitation from dorsal root ganglia tissue following axonal injury. The approach can be used to identify specific transcription factor binding sites and epigenetic modification of histone and DNA important for the regeneration of injured axons in both the peripheral and central nervous system.
Tags
Chromatin ImmunoprecipitationDorsal Root GangliaAxonal InjuryCentral Nervous SystemPeripheral Nervous SystemNeurite OutgrowthAxonal RegenerationTranscriptional ProgramsGene RegulationInjury Model SystemTranscriptional ActivityAnimal RemovalNerve FibersSciatic NerveSpinal CordTranscription Factor BindingHistone ModificationDNA ModificationChIP Protocol
