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Determinazione della mitocondriale Oxygen Species potenziale di membrana e reattiva in neuroni corticali di ratto dal vivo
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
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Determinazione della mitocondriale Oxygen Species potenziale di membrana e reattiva in neuroni corticali di ratto dal vivo

63,972 Views

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09:56 min

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May 23, 2011

DOI:

10.3791/2704-v

DOI:
10.3791/2704-v

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09:56 min
May 23, 2011

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10 Views
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1Department of Molecular Pharmacology and Experimental Therapeutics,Loyola University Chicago

Tags

Mitochondrial Membrane PotentialReactive Oxygen SpeciesLive Rat Cortical NeuronsRespiratory ChainATP GenerationOxidative StressEnvironmental ToxinsOxidative PhosphorylationMitochondrial DysfunctionFluorescent ProbesRhodamine 123TMRMTMREJC-1Fluorescence IndicatorsDihydroethidiumDihydrorhodamine 123H2DCF-DAFluorescence Intensity

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Joshi, D. C., Bakowska, J. C. Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons. J. Vis. Exp. (51), e2704, doi:10.3791/2704 (2011).

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