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小鼠视网膜神经节细胞的转染在体内电穿孔

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Neurociencias

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JoVE Revista Neurociencias

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation

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小鼠视网膜神经节细胞的转染在体内电穿孔

DOI:

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05:26 min

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April 17, 2011

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Onkar S. Dhande², Michael C. Crair

¹Department of Neurobiology,Yale University, ²Program in Developmental Biology,Baylor College of Medicine

Capítulos

00:05Título
01:24Equipment Set-up for in vivo Electroporation
02:08Micropipette and Injector Set-up for Retinal Microinjection
02:44Retinal Injection and Electroporation
04:06Single Retinal Ganglion Cell Labeling with EGFP Plasmid Constructs
05:00Conclusion

Summary

Traducción Automática

我们展示一个<em>在体内</em>电转染视网膜神经节细胞（RGC的）和其他类型的细胞在出生后的小鼠的年龄广泛视网膜的单件或小簇协议。标签和基因操纵产后视网膜神经节细胞的能力<em>在体内</em>是一个发展研究的有力工具。

Tags

Transfection Mouse Retinal Ganglion Cells In Vivo Electroporation RGC Projections Neural Connectivity Retinofugal Projections Lateral Geniculate Nucleus Superior Colliculus Developmental Changes Axonal Arbor Morphology Retinotopic Map Formation Genetic Manipulation Electroporation Protocols Postnatal Mice Labeling Efficiency

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