Journal
/
Medicina
/
Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents
JoVE Revista
Medicina
This content is Free Access.
JoVE Revista Medicina
Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents

Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents

18,334 Views

•

07:10 min

•

March 07, 2011

DOI:

10.3791/2558-v

DOI:
10.3791/2558-v

•

07:10 min
March 07, 2011

•

1 Views
, , ,
1Department of Pathology,University of Alabama at Birmingham – UAB, 2Department of Radiology,University of Alabama at Birmingham – UAB, 3Department of Cell Biology and Neurobiology,University of Alabama at Birmingham – UAB

Summary

Automatically generated

A method for reliably grafting luciferase-tagged human malignant peripheral nerve sheath tumor cells into the sciatic nerve of immunodeficient mice is described. The use of bioluminescence imaging to demonstrate proper establishment of tumor grafts and criteria for random segregation of animals into study groups are also discussed.

Explore More Videos

Orthotopic XenograftingHuman Malignant Peripheral Nerve Sheath Tumor CellsIn Vivo TestingCandidate Therapeutic AgentsAnimal ModelPreclinical TrialsTransgenic MiceXenograft ModelsImmunodeficient MiceTherapeutic Ranges

Read Article

Cite this Article

Turk, A. N., Byer, S. J., Zinn, K. R., Carroll, S. L. Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents. J. Vis. Exp. (49), e2558, doi:10.3791/2558 (2011).

Download .ris file

Copy

Share Video

.

Copy