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Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents
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Medizin
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JoVE Journal Medizin
Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents

Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents

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07:10 min

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March 07, 2011

DOI:

10.3791/2558-v

DOI:
10.3791/2558-v

•

07:10 min
March 07, 2011

•

1 Views
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1Department of Pathology,University of Alabama at Birmingham – UAB, 2Department of Radiology,University of Alabama at Birmingham – UAB, 3Department of Cell Biology and Neurobiology,University of Alabama at Birmingham – UAB

Summary

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A method for reliably grafting luciferase-tagged human malignant peripheral nerve sheath tumor cells into the sciatic nerve of immunodeficient mice is described. The use of bioluminescence imaging to demonstrate proper establishment of tumor grafts and criteria for random segregation of animals into study groups are also discussed.

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Turk, A. N., Byer, S. J., Zinn, K. R., Carroll, S. L. Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents. J. Vis. Exp. (49), e2558, doi:10.3791/2558 (2011).

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