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Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats
 

Changes in Mammary Gland Morphology and Breast Cancer Risk in Rats

Article DOI: 10.3791/2260-v • 09:36 min • October 16th, 2010
October 16th, 2010 •
1, 1,2, 1, 1
1Department of Oncology, Georgetown University, 2Institute of Biomedicine, University of Turku Medical Faculty

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Our protocol describes how to dissect the rat abdominal mammary gland and how to prepare mammary gland whole mounts. It also describes how to analyze mammary gland morphology using three end-points (number of terminal end buds, epithelial elongation and differentiation) and to use these results to predict mammary cancer risk in rats which were exposed to dietary modifications in utero or during prepuberty.

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Mammary Gland Morphology Breast Cancer Risk Rats Dietary Factors Hormonal Factors In Utero Pubertal Periods Modeling And Re-modeling Susceptibility To Carcinogen-induced Tumors High Birthweight Soy Intake Prenatal And Postnatal Dietary Modifications Persistent Morphological Changes Epigenetic Modifications DNA Methylation Histones MiRNA Expression Gene Transcription Dissecting And Removing Abdominal Mammary Gland Preparing Mammary Gland Whole Mounts Analyzing Mammary Gland Morphology Terminal End Buds Epithelial Elongation And Differentiation Predicting Risk Of Developing Mammary Cancer
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