Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
46,375 Views
•
08:47 min
•
November 17, 2010
DOI:
Summary
Automatically generated
ここでは、視覚野の神経細胞とグリア細胞の蛍光標識用AAVベクターの頭蓋内注射を提示する。
Explore More Videos
Intracranial InjectionAdeno-associated Viral VectorsLabeling TechniqueVisualizationSpecific Subsets Of CellsBrain RegionsIn VivoBrain SectionsFluorescent ProteinViral LabelingIndividual Cell TypesTransgenic Mouse LinesAAV VectorStereotaxic CoordinatesMicropipetteAutomated PumpPrecise DeliveryMinimal DamageInjection ParametersAnimal Age At InjectionInjection LocationVolume Of InjectionRate Of InjectionAAV SerotypePromoter Driving Gene ExpressionVirally-induced Transgene ExpressionVisualization Of Cells And ProcessesDendritic Spines
Read Article
Cite this Article
Lowery, R. L., Majewska, A. K. Intracranial Injection of Adeno-associated Viral Vectors. J. Vis. Exp. (45), e2140, doi:10.3791/2140 (2010).
Copy