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Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques

Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques

20,897 Views

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07:11 min

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April 22, 2010

DOI:

10.3791/1743-v

DOI:
10.3791/1743-v

•

07:11 min
April 22, 2010

•

1 Views
, , ,
1Department of Immunology,MD Anderson Cancer Center – University of Texas, 2Department of Medicine,University of Miami

Summary

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We demonstrate the utility of multicolor flow cytometry for detailed phenotypic and functional characterization of total as well as memory subsets of CD4+ and CD8+ T cells in rhesus macaques, the ideal model for HIV/AIDS vaccine studies.

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Multicolor Flow CytometryCellular Immune ResponseRhesus MacaquesHIV-AIDS ModelPathogenesisVaccinesTherapeuticsPhenotypic AnalysisFunctional AnalysisIntracellular Cytokine ProductionCD4+ T CellsCD8+ T CellsMemory SubsetsInterferon Gamma (INF-)Interleukin (IL)-2PMA Plus Ionomycin (PMA+I)Cytokine ProfilesMemory Cells SubsetsAntigen-specific CD4+ T CellsAntigen-specific CD8+ T CellsPBMC SamplesHIV Envelope Peptide Cocktail Vaccine

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He, H., Courtney, A. N., Wieder, E., Sastry, K. J. Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques. J. Vis. Exp. (38), e1743, doi:10.3791/1743 (2010).

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