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Obtener altamente purificada Toxoplasma gondii Los ooquistes por un gradiente de cloruro de cesio discontinua
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
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Obtener altamente purificada Toxoplasma gondii Los ooquistes por un gradiente de cloruro de cesio discontinua

13,224 Views

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11:17 min

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November 03, 2009

DOI:

10.3791/1420-v

DOI:
10.3791/1420-v

•

11:17 min
November 03, 2009

•

13 Views
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1Dynamac, Inc., 2Department of Biological Sciences,University of Cincinnati, McMicken College of Arts and Science, 3Animal Parasitic Disease Laboratory,Agricultural Research Service, U.S. Department of Agriculture, 4National Exposure Research Laboratory,US Environmental Protection Agency

Summary

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Este estudio describe el desarrollo de un método modificado de CsCl que fácilmente se purifica T. gondii ooquistes en las heces de gatos infectados que son adecuados para la biología molecular y la manipulación de cultivo de tejidos

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Cite this Article

Staggs, S. E., See, M. J., Dubey, J. P., Villegas, E. N. Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient. J. Vis. Exp. (33), e1420, doi:10.3791/1420 (2009).

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