Generation of Mice Derived from Induced Pluripotent Stem Cells

This method was used in the research reported in Boland et al. Nature. 461, 91-96 (2009).¹

1. Preparation of Lentivirus

This protocol employs doxycycline-inducible lentiviral shuttle vectors that encode for Oct4, Sox2, Klf4, and c-Myc under control of a tetO response element. Transgenes are activated by the reverse tetracycline trans-activating protein, rtTAM2.2¹⁶, which induces reprogramming factor expression in the presence of doxycycline. This system allows for tightly controlled, high expression of reprogramming factors. The lentiviral vectors used here are self-inactivating and thus cannot replicate following genomic integration. However, caution is required when working with lentiviruses and should be performed in laboratories compliant with BSL2 (USA) and S2 (Europe) standards.

1. Thaw HEK293T cells and passage at least once before transfection. Cells should be maintained at subconfluent density in HEK medium at 37 °C, 5% CO₂ in a humidified environment. Routinely passage cells every 2 days with a split ratio of 1:6-1:10.
2. Seed ~8 x 10⁶ HEK293T cells/T150 with 25 ml HEK medium. Use one T150 for each lentivirus preparation.
3. The following day transfect HEK293T cells by calcium phosphate precipitation. (Note: in our hands calcium phosphate precipitation routinely results in 80-90% transfection efficiency; however, cationic lipid transfection reagents such as Lipofectamine 2,000 may also be used). Prepare two 15 ml conical tubes for each virus to be prepared. Label the tubes “A” and “B”. Tube A, 10 μg of each of: the lentiviral shuttle vector encoding reprogramming factors (or rtTAM2.2), the viral packaging vectors, and plasmid encoding the viral envelope protein, VSVg. Add 186 μl 2 M CaCl₂ to Tube A, and bring the volume to 1.5 ml with sterile H₂O. Tube B: 1.5 ml 2x HBS (pre-warmed to room temperature).
4. Pipette the mixture in Tube A until it is a homogenous solution. Add solution A to solution B dropwise and let stand at room temperature for 2-3 min.
5. Aspirate growth medium of HEK293T cells and replace with 22 ml pre-warmed HEK medium without penicillin and streptomycin.
6. Pipette combined solutions AB (calcium phosphate precipitate) directly to HEK293T cells and distribute evenly by gentle rocking.
7. 24 hr after transfection of HEK-293T cells with lentivirus, remove growth medium and replace it with 25 ml of fresh, pre-warmed HEK medium. Return transfected HEKs to the incubator.
8. 48 hr after transfection of HEK-293T cells with lentivirus, collect growth media containing lentiviral particles from the transfected HEKs. Remove particulate debris from the harvested viral solution by centrifugation at 3,000 x g for 5 min at 4 °C.
9. Concentrate virus by ultracentrifugation through a 20% sucrose cushion (2 ml sucrose/25 ml viral supernatant) for 2 hr at 112,000 x g at 4 °C. Suspend viral pellet in 0.4 ml MEF medium at 4 °C for 15-30 min with gentle rocking. Store viral particles in single use aliquots (i.e. 50 μl) at -80 °C.

2. Preparation of Mouse Embryonic Fibroblasts (MEF) for Reprogramming

Note: The protocol outlined here relates to the derivation of iPSCs from E 13.5 mouse embryonic fibroblasts for use in TEC assays. While other groups have generated all-iPSC mice from adult donor cell sources, we have not tested this method on other cell types and cannot be certain that donor cell type is not a factor.

1. Set up mouse timed matings. On embryonic day 13.5 (E13.5), euthanize the pregnant female and dissect the embryos from the uterine horns. Place and store embryos in 1x PBS (pre-chilled to 4 °C) on ice.
2. Remove the extraembryonic tissues (i.e. chorion, amnion and placenta). Decapitate the embryo and remove the tail (optional – if needed for genotyping) and limbs. Scoop out the internal organs, using forceps or a scoop shaped spatula and mince the remaining carcass with the blade of a scalpel or sharp scissors.
3. Wash the minced carcass in 5 ml pre-chilled 1x PBS. Centrifuge at 200 x g for 5 min.
4. Aspirate the supernatant. Suspend pellet in 5 ml 0.25% Trypsin-EDTA and incubate at 37 °C with vigorous shaking for 20-30 min.
5. Add 5 ml of MEF medium (pre-warmed to 37 °C), mix and centrifuge at 200 x g for 5 min.
6. Aspirate supernatant and resuspend pellet in pre-warmed MEF medium.
7. Plate dissociated MEFs in 2-3 wells of a 6-well plate pre-coated with 0.1% gelatin. This is considered passage 1.
8. Passage MEFs at a dilution of 1:4-1:5 every 48 hr. MEFs at passage 3 are ready for lentiviral transduction.

3. Derivation of iPSC Lines

1. The day before lentiviral transduction; seed ~3 x 10⁵ primary MEFs into one well of a 6-well plate pre-coated with 0.1% gelatin.
2. Day 1: Primary MEFS should be 80-90% confluent for lentiviral transduction. Add lentiviral particles directly to MEF media and incubate with MEFs overnight at 37 °C, 5% CO₂ in a humidified environment.
3. The following day (day 2) aspirate the medium and wash twice with 3 ml of 1x PBS to remove viral particles.
4. Add 0.5 ml pre-warmed 0.25% Trypsin-EDTA to the cells and incubate at 37 °C for 3-5 min with occasional rocking.
5. Triturate to achieve a single-cell suspension. Observe cells by light microscopy to ensure a single cell suspension.
6. Transfer MEFs into a 15 ml conical tube containing 5 ml MEF media. Centrifuge at 200 x g for 5 min. Aspirate the supernatant and gently resuspend cells in MEF medium.
7. Evenly split the cell suspension between two wells of a 6 well plate pre-coated with 0.1% gelatin.
8. Rock the plate back and forth, side to side, and once in a circular motion to achieve an even distribution of cells throughout the well. Incubate overnight at 37 °C, 5% CO₂ in a humidified environment.
9. Day 3: Repeat steps 4-9 except split the cells evenly from one well to 3 wells of a 6 well plate pre-coated with 0.1% gelatin. This will yield 6 wells of transduced primary fibroblasts.
10. Day 4: Add doxycycline (dox) at a concentration of 10 μg/ml to 5/6 wells. One well should remain untreated to serve as a control.
11. Add VPA at 1.9 mM to 3 of the 5 wells treated with dox. VPA reduces the proliferation rate of MEFs. Dense cultures of MEFs tolerate prolonged exposure to VPA whereas subconfluent cultures tend to senesce within 2-5 days. Therefore, MEFS should be 100% confluent when VPA is added. Note: We use VPA in our reprogramming experiments because it is a known epigenetic modifier, and has been shown to increase the efficiency of iPSC generation¹⁷ although the effects and mechanisms of VPA action with respect to generating fully pluripotent iPSC lines are not known.
12. Day 5: Aspirate the medium, wash and trypsinize the cells as before. Passage the cells treated with dox/VPA to a 15 cm² tissue culture dish pre-coated with 0.1% gelatin and each of the other conditions to pre-coated 10 cm² dishes in ES cell medium supplemented with fresh dox and VPA.
13. Replenish cells every day with ESC medium supplemented with fresh dox and VPA. ESC-like colonies should begin to appear after ~7 days in the dox/VPA treatment and after ~10 days in the dox alone treatment. No colonies should appear in the absence of dox treatment.
14. Once colonies possess a bright refractive, well-defined border and contain 30-50 cells, manually isolate the colonies with a gel loading pipette tip and transfer to a U-bottom 96 well plate containing 20 μl of 0.25% trypsin-EDTA. Trypsinize to single cells and transfer to feeders in a flat bottom 96 well plate in 150 μl ESC medium containing dox/VPA or dox alone.
15. Continue to clonally expand the isolated iPSC lines on feeders in ESC medium. Remove dox and VPA on day 19 after their addition (post-transduction day 23). Discard iPSC lines that do not maintain self-renewal or proliferation rates similar to ESC controls.

It may be helpful to characterize your iPSC lines in relation to ESCs before attempting to perform TEC. We have characterized our lines by 1) expression of endogenous pluripotency markers (SSEA-1, Oct4, Sox2, Nanog) by immunocytochemistry, 2) karyotype analysis by chromosome counting and 3) embryoid body formation. One may also perform lentiviral-specific RT-qPCR to confirm that the proviral transgenes are not expressed in the iPSCs. However, we have identified fully pluripotent iPSCs using only morphology, immunostaining and karyotyping. In our experiments, selection of iPSC lines based on ESC-like morphology and growth characteristics results in the majority of the lines expressing pluripotency markers while we typically identify several lines with potentially abnormal karyotypes.

4. Preparation of iPSCs for Blastocyst Injection

Passage number of a PSC line has been shown to affect its pluripotency¹⁸ although this may be line dependent¹⁹. We have used iPSCs of from passages ^8-14 to produce adult all-iPSC mice.

1. Thaw iPSCs and plate on feeders in ESC medium. Passage the cells at least once on feeders before use for injection.
2. One well of a 6-well plate containing 70-80% confluent iPSCs will provide more than a sufficient number of cells for injection. Aspirate growth medium and wash the cells with ~3 ml 1x PBS (without Ca²⁺/Mg²⁺).
3. Add 0.5 ml pre-warmed 0.05% Trypsin-EDTA to the cells and incubate at 37 °C for 10 min with occasional rocking.
4. Triturate to achieve a single-cell suspension. Observe cells by light microscopy to ensure a single cell suspension. The iPSCs need to be in single cell suspension as colonies/cell aggregates will clog the injection pipette.
5. Once a single cell suspension has ben achieved, add 1.0 ml ESC media to the well and return the plate to the 37 °C incubator. Incubate for ~15 min or until the majority of feeders have begun to adhere.
6. Gently remove the medium containing the iPSCs taking care not to dislodge the weakly adherent feeders.
7. Place the iPSCs in a 15 ml conical tube containing 5 ml ESC medium. Centrifuge at 200 x g for 5 min. Aspirate the supernatant and remove the remainder of ES cell medium with a micropipette. Tap the tube to dislodge the pellet and gently resuspend cells in 0.2-0.5 ml pre-chilled FHM medium. Store cells on ice until and during injection into tetraploid blastocysts.

5. Generation of Tetraploid Blastocysts

Procedures performed in this section have been described in detail elsewhere^5,6,20. Here we outline our technique, optimized for the BTX Electro Cell Manipulator ECM 2001.

1. Set up embryo donor mice by priming 23-28 day old female mice (C57BL/6J-Tyr^c-2J /BALB/cByJ F1) with PMS and HCG. Administer 5 IU of PMS at 2PM and 5 IU of HCG 47 hr later. After HCG injection, set up female mice with C57BL/6J-Tyr^c-2J /BALB/cByJ F1 stud males. Check the following day for vaginal plugs.
2. Euthanize plugged female mice and collect oviducts. Collect 1-cell embryos by placing oviducts in FHM with Hyaluronidase and gently tearing the ampulae. Allow the cumulus masses to sit in FHM/Hyaluronidase for 5-7 min.
3. Collect 1-cell embryos using a mouth pipette and wash through drops of FHM media before placing them in KSOM-AA culture. Culture at 37 °C, 5% CO₂ under mineral oil overnight and select 2-cell embryos the day of electrofusion, discard all other embryos.
4. Place a BTX Microslide in a 10 cm Petri dish. Pour enough room temperature electrofusion media to submerge the slide in the solution, but not so much that the poles of the electrode are completely submerged.
5. Switch on ECM 2001 and BTX Enhancer 400. Connect the ECM’s cables to the microslide’s electrode and fix the cables to the side of the Petri dish to prevent unintended movement of the slide.
6. Run one manual pulse to get a reading on the BTX enhancer and note the voltage of the AC/DC currents being applied. AC current will control the speed at which the embryos will align between the electrodes, DC current will fuse the blastomeres, and pulse time will set the length of the DC pulse. A good starting point is AC 3V, DC 100V, and time 0.05 msec. The optimal DC varies in the range of 90-150 volts.
7. Using a mouth pipette, take about 30-40 two-cell embryos from KSOM-AA culture and wash them through several drops of electrofusion medium. Draw fresh electrofusion media from the microslide dish into mouth pipette and take embryos from the wash. Place them in the 1 mm gap between the electrodes on the microslide. Be careful that they are aligned down the middle of the gap and that they are not in contact with each other.
8. Apply AC current by pressing the manual pulse button. The embryos will rotate in the AC field, until the plane of blastomere contact is parallel to the electrodes. If embryos are not aligned in a few seconds, increase AC setting.
9. After embryos have aligned, press the manual pulse button again to apply the DC pulse.
10. With electrofusion medium in the pipette, collect the embryos from the microslide. Wash embryos through several drops of KSOM-AA and place them in KSOM-AA culture at 37 °C, 5% CO₂. The blastomere fusion should be completed in less than 30 min in culture.
11. Repeat steps 7-11 for remaining 2-cell embryos. After subsequent fusion groups, monitor and select embryos with fused blastomeres. Successfully fused embryos will appear to be in 1-cell stage. Discard lysed and 2-cell embryos after 30 min in culture. If fusion rate is below 80%, increase voltage and/or time in increments of 5V and 0.01 msec. If lysis is above 20%, decrease DC voltage and/or time accordingly. The optimal settings in our experiments were AC 4V, DC 146V, and 0.07 msec. These settings consistently yielded 90% or higher fusion rates with little or no lysis.
12. Continue to culture fused embryos in microdrops of KSOM-AA under mineral oil at 37 °C, 5% CO₂. You should expect 85-95% of fused embryos to form tetraploid (4n) blastocysts after 48 hr of incubation.

6. Microinjection of iPSCs into Tetraploid Blastocysts

We use a Nikon TE-2000U inverted microscope equipped with DIC optics and Narishige micromanipulators for blastocyst injection. Each tetraploid blastocyst is injected with 10-12 iPSCs using a standard protocol for ESC injection into mouse blastocysts that has been demonstrated in a previous JoVE publication^5,20,21

1. Place a 20 μl drop of FHM in the center of a concave microscope slide and cover it with 150 μl of mineral oil.
2. Lower the holding pipette and microinjection needle into the FHM drop. Allow 2-3 min for both needles to partially fill with FHM.
3. Wash 20-30 tetraploid blastocysts through drops of FHM and transfer to the FHM drop on the microscope slide.
4. Mouth pipette iPSC mixture into the drop. It may be necessary to dilute the cell mixture in a drop of FHM beforehand if cells are too concentrated or aggregated.
5. Pick up 100-200 cells with the injection needle.
6. Hold the blastocyst with the inner cell mass in the 9 o’clock position. Inject cells into blastocoel by penetrating the zona pellucida and trophoblast at the 3 o’clock position. Inject 16-18 cells per blastocyst.
7. Return iPSC complemented blastocysts to KSOM-AA culture.

7. Transfer of Complemented Tetraploid Blastocysts into the Uterine Horns of Recipient Mice

Complemented tetraploid blastocysts are surgically transferred to the uterine horns of female recipient mice according to the guidelines of the researcher’s institute, using the standard technique²⁰ which we shall briefly summarize. Select female CD-1 mice at the pro-estrus stage and set them up for mating with vasectomized males. Check for vaginal plugs the next morning. Females are ready for uterine embryo transfer two days after the plug was detected (2.5 dpc).

One day before recipient females are mated with vasectomized males, set up additional CD-1 females with non-vasectomized males to be used as foster mothers for all-iPSC mice retrieved by Caesarian section.

8. Caesarean Section and Fostering of iPSC-derived Pups

The transfer of TC embryos typically results in multiple resorptions after the implantation, even if the iPSC or ESC line has a high developmental potential. As a result, one can expect not more than 4 viable pups (usually 1-2) per recipient. These small litters are usually neglected by recipients. To increase the level of neonatal care and the rate of survival, we perform C-sections and fostering according to the standard protocols²⁰. To perform the Caesarean section, euthanize recipient mice 16 days after embryo transfer at 7-8PM (recipient 18.5 dpc) and dissect pups from the uterine horns. Foster viable pups to CD-1 mothers that delivered litters the same day.