Intracerebroventricular Injection in Mouse: An Ommaya Mediated Direct Drug Administration Method to Deliver Drugs to Cerebral Ventricles of Mouse Brain

Published: April 30, 2023

Abstract

Source: Law, V. et al. A Murine Ommaya Xenograft Model to Study Direct-Targeted Therapy of Leptomeningeal Disease. J. Vis. Exp. (2021)

This video demonstrates ommaya mediated intracerebroventricular injection in a mouse model. The method enables localized delivery of potential drug candidates and evades the blood-brain barrier.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Murine Ommaya Assembly and Implantation Preparation of station Disinfect the station surface. Place a blue covering over the surface and keep ready all sterilized tools and supplies indicated in Figure 1. Presurgical procedure Apply analgesia (Bup-SR)…

Representative Results

Figure 1: An example of a workstation setup for performing the Murine Ommaya implantation in mice. (1) Gas anesthesia machine/vaporizer. (2) Sterile blue paper drape covering a stereotaxic stand. (3) Stereotaxic device (stand/stage, ear bars, nose cone). (4) Microdrill. (5) Magnifying glass with light. (6) Sterile cotton-tipped applicator sticks with sterile saline rinse container. (7) Hydrogen peroxide. (8) Bead sterilizer. <img alt="Fi…

Divulgaciones

The authors have nothing to disclose.

Materials

1 mm spacer disc Alzet, Durect Corporation #0008670 Spacer disc only
4-0 ethilon nylon suture Any vendor
Automatic syringe pumps Harvard Syringe Pumps (or any vendor) #70-4505 Pump 11 Elite
Bead sterilizer Braintree Scientific Inc. (or any vendor) #GER 5287-120V Germinator 500
Buprenorphine Sustained-Release (Bup-SR) Zoopharm DEA controlled
Cyanoacrylate sterile adhesive Any vendor
Gas inhalation anestehsia system VeteEquip #901812 COMPAC5
Hamilton microliter syringes Hamilton 10, 25, 50, and 100 μL;
30G for cisterna magna injection
Hydrogen peroxide Any vendor
IVIS 200 imaging system Caliper Life Sciences
Magnifying glass with light Any vendor
Microdrill Stoelting (or any vendor) #51555M
MRI imaging Bruker BioSpec series Optional
Murine Ommaya (MRI-compatible) prototype Instech Laboratories, Inc. #VAB620-25MRI-3.3
PinPort injector Instech Laboratories, Inc. #PNP3M-50
PinPort Instech Laboratories, Inc. #1-PNP3F28-50
Rodent Surgical Instruments (Scissors, Forceps) Roboz Surgical Instrument (or any vendor)
Stereotaxic device Stoelting (or any vendor) #51730M
Sterile blue paper/ drape covering Any vendor
Sterile cotton sticks Any vendor

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Intracerebroventricular Injection in Mouse: An Ommaya Mediated Direct Drug Administration Method to Deliver Drugs to Cerebral Ventricles of Mouse Brain. J. Vis. Exp. (Pending Publication), e20612, doi: (2023).

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