Intracerebroventricular Injection in Mouse: An Ommaya Mediated Direct Drug Administration Method to Deliver Drugs to Cerebral Ventricles of Mouse Brain
Published: April 30, 2023
Abstract
Source: Law, V. et al. A Murine Ommaya Xenograft Model to Study Direct-Targeted Therapy of Leptomeningeal Disease. J. Vis. Exp. (2021)
This video demonstrates ommaya mediated intracerebroventricular injection in a mouse model. The method enables localized delivery of potential drug candidates and evades the blood-brain barrier.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Murine Ommaya Assembly and Implantation Preparation of station Disinfect the station surface. Place a blue covering over the surface and keep ready all sterilized tools and supplies indicated in Figure 1. Presurgical procedure Apply analgesia (Bup-SR)…
Representative Results
Figure 1: An example of a workstation setup for performing the Murine Ommaya implantation in mice. (1) Gas anesthesia machine/vaporizer. (2) Sterile blue paper drape covering a stereotaxic stand. (3) Stereotaxic device (stand/stage, ear bars, nose cone). (4) Microdrill. (5) Magnifying glass with light. (6) Sterile cotton-tipped applicator sticks with sterile saline rinse container. (7) Hydrogen peroxide. (8) Bead sterilizer. <img alt="Fi…
Divulgaciones
The authors have nothing to disclose.
Materials
| 1 mm spacer disc | Alzet, Durect Corporation | #0008670 | Spacer disc only |
| 4-0 ethilon nylon suture | Any vendor | ||
| Automatic syringe pumps | Harvard Syringe Pumps (or any vendor) | #70-4505 | Pump 11 Elite |
| Bead sterilizer | Braintree Scientific Inc. (or any vendor) | #GER 5287-120V | Germinator 500 |
| Buprenorphine Sustained-Release (Bup-SR) | Zoopharm | DEA controlled | |
| Cyanoacrylate sterile adhesive | Any vendor | ||
| Gas inhalation anestehsia system | VeteEquip | #901812 | COMPAC5 |
| Hamilton microliter syringes | Hamilton | 10, 25, 50, and 100 μL; 30G for cisterna magna injection |
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| Hydrogen peroxide | Any vendor | ||
| IVIS 200 imaging system | Caliper Life Sciences | ||
| Magnifying glass with light | Any vendor | ||
| Microdrill | Stoelting (or any vendor) | #51555M | |
| MRI imaging | Bruker | BioSpec series | Optional |
| Murine Ommaya (MRI-compatible) prototype | Instech Laboratories, Inc. | #VAB620-25MRI-3.3 | |
| PinPort injector | Instech Laboratories, Inc. | #PNP3M-50 | |
| PinPort | Instech Laboratories, Inc. | #1-PNP3F28-50 | |
| Rodent Surgical Instruments (Scissors, Forceps) | Roboz Surgical Instrument (or any vendor) | ||
| Stereotaxic device | Stoelting (or any vendor) | #51730M | |
| Sterile blue paper/ drape covering | Any vendor | ||
| Sterile cotton sticks | Any vendor |
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Intracerebroventricular Injection in Mouse: An Ommaya Mediated Direct Drug Administration Method to Deliver Drugs to Cerebral Ventricles of Mouse Brain. J. Vis. Exp. (Pending Publication), e20612, doi: (2023).