Source: Flesken-Nikitin, A. et al. Transplantation Into the Mouse Ovarian Fat Pad. J. Vis. Exp. (2016)
In this video, we demonstrate an assay for transplantation of experimental cells and tissue fragments into the mouse ovarian fat pad. This method can be used to monitor cell regeneration and tumor transformation via non-invasive techniques.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Ovarian Fat Pad Transplantation
NOTE: Disinfect instruments between each animal surgery. Prepare and arrange all equipment in advance. Dorsal transplantations to the right ovarian fat pad are preferential as this avoids the spleen. However, recipients may have transplants in both ovarian fat pads.
The authors have nothing to disclose.
28 gauge needle (hypodermic syringe with attached needle) | Kendall | 30339 | Part Number: 8881500014 |
Basement membrane matrix (Geltrex) |
Invitrogen | A1413202 | |
Ethanol 200 proof | Koptec | V1001 | To prepare 70% |
Filter tip 0.1-10/20 µl XL | USA Scientific | 1120-3810 | |
Isoflurane, 250 ml | Santa Cruz Animal Health | sc-363629Rx | |
Ketoprofen | Zoetis | 4396H | |
Microscope, stereo | Nikon | SMZ800 | |
Nod/SCID/gamma (NSG) mice | The Jackson Laboratory | 5557 | NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ |
severe combined immunodeficiency (SCID/NCr) mice, BALB/C background) |
NCI-Frederick Animal Production Program | 01S11 |