Ovarian Fat Pad Transplantation Assay: An In Vivo Technique to Introduce Cells into Ovarian Fat Pad in Mouse Models
Published: April 30, 2023
Abstract
Source: Flesken-Nikitin, A. et al. Transplantation Into the Mouse Ovarian Fat Pad. J. Vis. Exp. (2016)
In this video, we demonstrate an assay for transplantation of experimental cells and tissue fragments into the mouse ovarian fat pad. This method can be used to monitor cell regeneration and tumor transformation via non-invasive techniques.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Ovarian Fat Pad Transplantation NOTE: Disinfect instruments between each animal surgery. Prepare and arrange all equipment in advance. Dorsal transplantations to the right ovarian fat pad are preferential as this avoids the spleen. However, recipients may have transplants …
Divulgaciones
The authors have nothing to disclose.
Materials
| 28 gauge needle (hypodermic syringe with attached needle) | Kendall | 30339 | Part Number: 8881500014 |
| Basement membrane matrix (Geltrex) |
Invitrogen | A1413202 | |
| Ethanol 200 proof | Koptec | V1001 | To prepare 70% |
| Filter tip 0.1-10/20 µl XL | USA Scientific | 1120-3810 | |
| Isoflurane, 250 ml | Santa Cruz Animal Health | sc-363629Rx | |
| Ketoprofen | Zoetis | 4396H | |
| Microscope, stereo | Nikon | SMZ800 | |
| Nod/SCID/gamma (NSG) mice | The Jackson Laboratory | 5557 | NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ |
| severe combined immunodeficiency (SCID/NCr) mice, BALB/C background) |
NCI-Frederick Animal Production Program | 01S11 |
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Ovarian Fat Pad Transplantation Assay: An In Vivo Technique to Introduce Cells into Ovarian Fat Pad in Mouse Models. J. Vis. Exp. (Pending Publication), e20383, doi: (2023).