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Journal
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Entwicklungsbiologie
/
Comparative Analysis of Forward and Reverse Transfection Methods on mPSCs
/
Author Spotlight: Advancements in Synthetic Genetic Devices for Stem Cell Fate Manipulation and Cellular Therapy Development
JoVE Journal
Entwicklungsbiologie
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JoVE Journal
Entwicklungsbiologie
An Optimized Mouse Embryonic Stem Cell Based Reverse Poly-Transfection Technique for Rapid Exploration of Nucleic Acid Ratios
Author Spotlight: Advancements in Synthetic Genetic Devices for Stem Cell Fate Manipulation and Cellular Therapy Development
Comparative Analysis of Forward and Reverse Transfection Methods on mPSCs
DOI:
10.3791/65766-v
•
01:23 min
•
December 08, 2023
•
Kieran Maheden
,
Karen Hwang
,
Ipek Egilmez
,
Nika Shakiba
1
School of Biomedical Engineering
,
University of British Columbia
Tags
Mouse Embryonic Stem Cells
Reverse Transfection
Poly-transfection
Nucleic Acid Ratios
Genetic Circuits
DNA Stoichiometry
Plasmid Delivery
2i/LIF Media
Regenerative Medicine
Biomedical Research
Synthetic Genetic Devices
Cell Fate
Cellular Therapies
Gene Expression
Gene Dosage
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Author Spotlight: Advancements in Synthetic Genetic Devices for Stem Cell Fate Manipulation and Cellular Therapy Development
Preparation of Mouse Pluripotent Stem Cells (mPSCs) for Transfection
Reverse and Forward Transfection of Mouse Pluripotent Stem Cells (mPSCs)
Flow Cytometry Analysis of Transfected Mouse Pluripotent Stem Cells (mPSCs)
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