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Comparative Analysis of Forward and Reverse Transfection Methods on mPSCs
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Author Spotlight: Advancements in Synthetic Genetic Devices for Stem Cell Fate Manipulation and Cellular Therapy Development
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发育生物学
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JoVE 杂志 发育生物学
An Optimized Mouse Embryonic Stem Cell Based Reverse Poly-Transfection Technique for Rapid Exploration of Nucleic Acid Ratios

Author Spotlight: Advancements in Synthetic Genetic Devices for Stem Cell Fate Manipulation and Cellular Therapy Development

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01:23 min

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December 08, 2023

DOI:

10.3791/65766-v

DOI:
10.3791/65766-v

•

01:23 min
December 08, 2023

•

14 Views
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1School of Biomedical Engineering,University of British Columbia

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Mouse Embryonic Stem CellsReverse TransfectionPoly-transfectionNucleic Acid RatiosGenetic CircuitsDNA StoichiometryPlasmid Delivery2i/LIF MediaRegenerative MedicineBiomedical ResearchSynthetic Genetic DevicesCell FateCellular TherapiesGene ExpressionGene Dosage

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Maheden, K., Hwang, K., Egilmez, I., Shakiba, N. An Optimized Mouse Embryonic Stem Cell Based Reverse Poly-Transfection Technique for Rapid Exploration of Nucleic Acid Ratios. J. Vis. Exp. (202), e65766, doi:10.3791/65766 (2023).

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