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Isolamento di vescicole extracellulari a basso contenuto di endotossine derivate da linee cellulari tumorali
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
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Isolamento di vescicole extracellulari a basso contenuto di endotossine derivate da linee cellulari tumorali

789 Views

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06:28 min

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February 17, 2023

DOI:

10.3791/65062-v

DOI:
10.3791/65062-v

•

06:28 min
February 17, 2023

•

4 Views
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1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Summary

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Il protocollo proposto include linee guida su come evitare la contaminazione con endotossina durante l'isolamento di vescicole extracellulari da supernatanti di coltura cellulare e su come valutarle correttamente.

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Cite this Article

Babula, A., Siemińska, I., Baj-Krzyworzeka, M. Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines. J. Vis. Exp. (192), e65062, doi:10.3791/65062 (2023).

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