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Hypothalamus kisspeptin nevroner som et mål for helcelle patch-klemme opptak
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JoVE Journal Neurowissenschaften
Hypothalamic Kisspeptin Neurons as a Target for Whole-Cell Patch-Clamp Recordings

Hypothalamus kisspeptin nevroner som et mål for helcelle patch-klemme opptak

DOI:
10.3791/64989-v

•

09:39 min

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March 17, 2023

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, , ,
1Department of Anatomy, Instituto de Ciencias Biomedicas,Universidade de Sao Paulo

Kapitel

  • 00:05Introduction
  • 00:48Brain Dissection and Slicing
  • 02:49Cell Sealing for Patch‐Clamp Recording
  • 07:29Results: Whole‐Cell Patch‐Clamp Recordings on the Brain Slices
  • 08:39Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Her presenterer vi en protokoll for å utføre en helcellelapp-klemme på hjerneskiver som inneholder kisspeptin nevroner, den primære modulatoren av gonadotropinfrigjørende hormon (GnRH) celler. Ved å legge til kunnskap om kisspeptin neuron aktivitet, har dette elektrofysiologiske verktøyet tjent som grunnlag for betydelige fremskritt i nevroendokrinologi feltet de siste 20 årene.

Tags

Keywords: Whole-cell Patch-clamp RecordingsElectrophysiological PropertiesCell MembranesIonic CurrentsCellular ResponseBrain DissectionHypothalamusOptic NerveVibratomeACSF SolutionMicroscopeAmplifierDigitizerMicromanipulatorPerfusion Pump

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