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3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens
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Kapitel
- 00:05Introduction
- 01:08Preparation of Plants for Glass Bottom Dishes and 3-D Image Reconstruction
- 03:19Results: 3-D Time Series of Moss Development
- 04:00Conclusion
This manuscript presents a detailed protocol to image the 3-D cell wall dynamics of living moss tissue, allowing the visualization of the detachment of cell walls in ggb mutants and thickening cell wall patterns in the wild type during development over a long period.
