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发育生物学
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3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens

3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens

DOI:
10.3791/64651-v

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05:14 min

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February 10, 2023

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,
1Department of Biology,University of Louisville

Chapters

  • 00:05Introduction
  • 01:08Preparation of Plants for Glass Bottom Dishes and 3-D Image Reconstruction
  • 03:19Results: 3-D Time Series of Moss Development
  • 04:00Conclusion

Summary

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This manuscript presents a detailed protocol to image the 3-D cell wall dynamics of living moss tissue, allowing the visualization of the detachment of cell walls in ggb mutants and thickening cell wall patterns in the wild type during development over a long period.

Tags

3D Time-lapse ImagingCell Wall DynamicsCalcofluor StainingPhyscomitrium PatensMossFluorescence MicroscopyBCDATG MediumGrid System For Sample Positioning

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