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Geração de células assassinas naturais a partir de células-tronco de potencial expandido humano
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Generation of Natural Killer Cells from Human Expanded Potential Stem Cells

Geração de células assassinas naturais a partir de células-tronco de potencial expandido humano

2,379 Views

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06:53 min

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January 13, 2023

DOI:

10.3791/64608-v

DOI:
10.3791/64608-v

•

06:53 min
January 13, 2023

•

11 Views
, , , ,
1Major in Molecular Biology and Biotechnology, School of Biological Sciences,The University of Hong Kong, 2School of Biomedical Sciences,The University of Hong Kong, 3Centre for Translational Stem Cell Biology

Summary

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O presente protocolo mostra como diferenciar células CD3−/CD45+CD56+ com citotoxicidade leve de células-tronco potenciais expandidas humanas (hEPSCs) em condições de cultura 3D e 2D. Isso permite a validação fenotípica de rotina sem a destruição do microambiente complexo.

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Cite this Article

Yu Ching, P., Wang, C., Hang, S., Liu, P., Sugimura, R. Generation of Natural Killer Cells from Human Expanded Potential Stem Cells. J. Vis. Exp. (191), e64608, doi:10.3791/64608 (2023).

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