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Isolamento, attivazione ed espansione delle cellule T basate sulla flottazione da campioni di cellule mononucleate del sangue periferico umano utilizzando microbolle
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Kapitel
- 00:04Introduction
- 00:29Isolation of T Cells with Microbubbles Using Positive Selection
- 01:51Co-Stimulation (Activation) of the Positively Selected T Cells
- 02:52Expansion of the Co-Stimulated Cells in Cell Culture Medium
- 03:28Expansion of the T Cells (With or Without Prior Transduction)
- 04:02Harvesting the T Cells and Flow Cytometry
- 05:16Results: Assessment of Viable and Successfully Transduced Cells
- 05:58Conclusion
