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Generering af retinale organoider fra sunde og retinale sygdomsspecifikke human-inducerede pluripotente stamceller
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Generering af retinale organoider fra sunde og retinale sygdomsspecifikke human-inducerede pluripotente stamceller

2,675 Views

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09:47 min

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December 09, 2022

DOI:

10.3791/64509-v

DOI:
10.3791/64509-v

•

09:47 min
December 09, 2022

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14 Views
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1Centre for Ocular Regeneration, Prof. Brien Holden Eye Research Centre,LV Prasad Eye Institute, 2Manipal Academy of Higher Education, 3Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School

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Retinal OrganoidsHuman Induced Pluripotent Stem CellsRetinal DegenerationRetinal Pigment EpitheliumIn Vitro ModelsCell-based TherapiesEye DevelopmentInherited Retinal DiseasesDifferentiationAdherent CultureSuspension CultureRetinal CupsSpatial PositioningMorphological FeaturesCell DissociationIPSC CultureDifferentiation Induction MediumBFGFNoggin

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Mahato, S., Agrawal, T., Pidishetty, D., Maddileti, S., Pulimamidi, V. K., Mariappan, I. Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells. J. Vis. Exp. (190), e64509, doi:10.3791/64509 (2022).

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