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脑室微注射脂多糖到斑马鱼幼虫中以评估神经炎症和神经毒性

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JoVE Journal Neurowissenschaften

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Please note that all translations are automatically generated. Click here for the English version.

脑室微注射脂多糖到斑马鱼幼虫中以评估神经炎症和神经毒性

DOI:

10.3791/64313-v

•

07:31 min

•

August 23, 2022

•

Yulin He, Simon Ming Yuen Lee²

¹State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences,University of Macau, Avenida da Universidade, ²Department of Pharmaceutical Sciences, Faculty of Health Sciences,University of Macau, Avenida da Universidade

Kapitel

00:05Introduction
00:39Preparing and Mounting Zebrafish for Microinjections
02:22Injecting the Brain Ventricle
03:08Imaging
03:43Determination of Gene Expression Markers
04:44Zebrafish Locomotive Behavioral Assay
05:34Results: Lipopolysaccharide-Induced Neuroinflammation to Assess the Levels of Proinflammatory Mediators
07:02Conclusion

Summary

Automatische Übersetzung

该协议展示了在斑马鱼幼虫模型中将脂多糖显微注射到脑室区域，以研究由此产生的神经炎症反应和神经毒性。

Tags

Keywords: Brain Ventricle Microinjection Lipopolysaccharide Neuroinflammation Neurotoxicity Zebrafish In Vivo Model Anti-neuroinflammatory Drugs Glass Capillary Micromanipulator Agarose Anesthesia

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