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小鼠胸腺细胞在δ样4表达基质细胞上的共培养和转导以研究T细胞白血病的癌基因
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09:43 min
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June 09, 2023
DOI:
1Cytokines and Immunity Section, Cancer Innovation Laboratory, National Cancer Institute,National Institutes of Health, 2Comparative Biomedical Scientist Training Program,NIH, 3Department of Pathobiology and Diagnostic Investigation, Veterinary Diagnostic Laboratory,Michigan State University, 4NCI-Frederick Laboratory Animal Sciences Program
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该协议描述了从小鼠胸腺中分离双阴性胸腺细胞，然后在δ样4表达骨髓基质细胞系共培养系统（OP9-DL4）上进行逆转录病毒转导和共培养以进行进一步的功能分析。
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Rodrigues, G. O. L., Li, W., Cramer, S. D., Winer, H. Y., Hsu, T. C., Gower, T., Hixon, J. A., Durum, S. K. Co-Culture and Transduction of Murine Thymocytes on Delta-Like 4-Expressing Stromal Cells to Study Oncogenes in T-Cell Leukemia. J. Vis. Exp. (196), e64271, doi:10.3791/64271 (2023).
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