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通过全内反射荧光（TIRF）显微镜 在体外 可视化肌动蛋白和微管耦合动力学
Kapitel
- 00:04Introduction
- 00:40Coating Cleaned Coverslips with mPEG- and Biotin-PEG-Silane
- 01:21Assembling Imaging Flow Chambers
- 02:50Conditioning of Perfusion Chambers
- 03:47Microscope Preparation
- 04:40Preparation of Protein Reaction Mixes
- 06:02Image Actin and Microtubule Dynamics
- 06:43Results: Example Measurements of Microtubules and Actin Filament Dynamics
- 08:14Conclusion
