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초저온 전자 현미경 구조 결정을 위한 DNA 복구 인자와 복합체화된 뉴클레오솜 코어 입자의 준비
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Biochemie
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JoVE Journal Biochemie
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
Please note that all translations are automatically generated. Click here for the English version.

초저온 전자 현미경 구조 결정을 위한 DNA 복구 인자와 복합체화된 뉴클레오솜 코어 입자의 준비

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07:59 min

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August 17, 2022

DOI:

10.3791/64061-v

DOI:
10.3791/64061-v

•

07:59 min
August 17, 2022

•

5 Views
, , , ,
1Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, National Institutes of Health

Summary

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이 프로토콜은 TEM 그리드 동결을 위한 두 가지 샘플 준비 방법을 사용하여 뉴클레오솜 복합체의 준비를 자세히 설명합니다.

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NucleosomeDNA RepairCryo-electron MicroscopySample PreparationCross-linkingSize Exclusion ChromatographyBuffer ExchangeProtein Complex

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Cite this Article

Rodriguez, Y., Butay, K. J., Sharma, K., Viverette, E., Wilson, S. H. Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination. J. Vis. Exp. (186), e64061, doi:10.3791/64061 (2022).

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