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Preparação de Partículas Nucleares Complexadas com Fatores de Reparo de DNA para Determinação Estrutural por Microscopia Crio-Eletrônica
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Biochemie
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JoVE Journal Biochemie
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Preparação de Partículas Nucleares Complexadas com Fatores de Reparo de DNA para Determinação Estrutural por Microscopia Crio-Eletrônica

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07:59 min

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August 17, 2022

DOI:

10.3791/64061-v

DOI:
10.3791/64061-v

•

07:59 min
August 17, 2022

•

5 Views
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1Genome Integrity and Structural Biology Laboratory,National Institute of Environmental Health Sciences, National Institutes of Health

Summary

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Este protocolo descreve em detalhes a preparação de complexos nucleossômicos usando dois métodos de preparação de amostras para congelamento de grades de TEM.

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NucleosomeDNA RepairCryo-electron MicroscopySample PreparationCross-linkingSize Exclusion ChromatographyBuffer ExchangeProtein Complex

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Cite this Article

Rodriguez, Y., Butay, K. J., Sharma, K., Viverette, E., Wilson, S. H. Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination. J. Vis. Exp. (186), e64061, doi:10.3791/64061 (2022).

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