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毫秒氢/氘交换质谱法用于生理条件下α-突触核蛋白结构动力学的研究
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JoVE Journal Neurowissenschaften
Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions
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毫秒氢/氘交换质谱法用于生理条件下α-突触核蛋白结构动力学的研究

2,573 Views

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08:40 min

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June 23, 2022

DOI:

10.3791/64050-v

DOI:
10.3791/64050-v

•

08:40 min
June 23, 2022

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6 Views
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1Living Systems Institute, Department of Biosciences,University of Exeter

Tags

Alpha-synucleinHydrogen/deuterium ExchangeMass SpectrometryIntrinsically Disordered ProteinsPeptide MappingSample PreparationAutosamplerHDX RoboticsQuench BufferPeptide Coverage MapFast HDX Prototype InstrumentHDX Data Analysis Software

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Seetaloo, N., Phillips, J. J. Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions. J. Vis. Exp. (184), e64050, doi:10.3791/64050 (2022).

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