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Sinpses de fita de imunolabeling e contagem em jovens adultos e cócleas de Gerbil Envelhecido
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JoVE Journal Neurowissenschaften
Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae

Sinpses de fita de imunolabeling e contagem em jovens adultos e cócleas de Gerbil Envelhecido

2,233 Views

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08:25 min

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April 21, 2022

DOI:

10.3791/63874-v

DOI:
10.3791/63874-v

•

08:25 min
April 21, 2022

•

5 Views
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1Cluster of Excellence “Hearing4all” and Research Centre Neurosensory Science, Department of Neuroscience, School of Medicine and Health Science,Carl von Ossietzky University Oldenburg

Summary

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Um protocolo para processar a cópula gerbil jovem e envelhecida imunolabelando as estruturas sinápticas e células ciliadas anciavelmente, saciando a autofluorescência no tecido envelhecido, dissecando e estimando o comprimento da cóclea, e quantificando as sinapses em pilhas de imagens obtidas com imagens confocalas.

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Cite this Article

Steenken, F., Bovee, S., Köppl, C. Immunolabeling and Counting Ribbon Synapses in Young Adult and Aged Gerbil Cochleae. J. Vis. Exp. (182), e63874, doi:10.3791/63874 (2022).

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