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Standaardisatie en onderhoud van 3D canine hepatische en intestinale organoïde culturen voor gebruik in biomedisch onderzoek
JoVE Journal
Medizin
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JoVE Journal Medizin
Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research
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Standaardisatie en onderhoud van 3D canine hepatische en intestinale organoïde culturen voor gebruik in biomedisch onderzoek

3,866 Views

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08:39 min

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January 31, 2022

DOI:

10.3791/63515-v

DOI:
10.3791/63515-v

•

08:39 min
January 31, 2022

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10 Views
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1Department of Biomedical Sciences, College of Veterinary Medicine,Iowa State University, 2Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University, 33D Health Solutions Inc., 4Department of Molecular Virology and Microbiology,Baylor College of Medicine

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Canine Hepatic OrganoidsCanine Intestinal OrganoidsOrganoid CultureBiomedical ResearchStandardizationMaintenanceIsolationHarvestingBiobankingExtracellular MatrixChelating SolutionDMEMGrowth Factors

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Gabriel, V., Zdyrski, C., Sahoo, D. K., Dao, K., Bourgois-Mochel, A., Kopper, J., Zeng, X., Estes, M. K., Mochel, J. P., Allenspach, K. Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research. J. Vis. Exp. (179), e63515, doi:10.3791/63515 (2022).

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