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使用透射电子显微镜定量骨骼肌纤维中的亚细胞糖原分布
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Biochemie
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JoVE Journal Biochemie
Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy
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使用透射电子显微镜定量骨骼肌纤维中的亚细胞糖原分布

3,735 Views

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08:32 min

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February 07, 2022

DOI:

10.3791/63347-v

DOI:
10.3791/63347-v

•

08:32 min
February 07, 2022

•

13 Views
, , , ,
1Research center for applied health science,University College South Denmark, 2Department of Sports Science and Clinical Biomechanics,University of Southern Denmark, 3Department of Biomedical Sciences, Core Facility for Integrated Microscopy,University of Copenhagen

Summary

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改进的固定后程序增加了组织中糖原颗粒的对比度。本文提供了一个分步方案，描述了如何处理组织，进行成像，并使用立体学方法获得骨骼肌中纤维类型特异性亚细胞糖原分布的无偏和定量数据。

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Jensen, R., Ørtenblad, N., di Benedetto, C., Qvortrup, K., Nielsen, J. Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy. J. Vis. Exp. (180), e63347, doi:10.3791/63347 (2022).

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