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DetectSyn：一种快速、无偏倚的荧光方法，用于检测突触密度的变化

JoVE Journal
Biochemie

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JoVE Journal Biochemie

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

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DetectSyn：一种快速、无偏倚的荧光方法，用于检测突触密度的变化

3,016 Views

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09:10 min

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July 22, 2022

DOI:

10.3791/63139-v

DOI:

10.3791/63139-v

•

09:10 min

July 22, 2022

•

3 Views

Chelcie F. Heaney^1,2, Colin J. McArdle², Kimberly F. Raab-Graham^1,2

¹Wake Forest Translational Alcohol Research Center,Wake Forest School of Medicine, ²Physiology and Pharmacology,Wake Forest School of Medicine

Summary

DetectSyn是一种无偏倚的快速荧光测定，可测量治疗或疾病状态下相对突触（突触前和突触后接合）数量的变化。该技术利用了一种接近连接技术，该技术既可用于培养的神经元，也可用于固定组织。

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