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Neuroscience

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JoVE Journal Neuroscience

小鼠大脑中人类神经祖细胞的脑内移植和体内生物发光跟踪

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小鼠大脑中人类神经祖细胞的脑内移植和体内生物发光跟踪

Article DOI: 10.3791/63102-v • 06:12 min • January 27th, 2022

January 27th, 2022 •

Rebecca Z. Weber^1,2, Chantal Bodenmann¹, Daniela Uhr¹, Kathrin J. Zürcher¹, Debora Wanner¹, Melanie Generali¹, Roger M. Nitsch¹, Ruslan Rust*^1,2, Christian Tackenberg*^1,2

¹Institute for Regenerative Medicine, University of Zurich, ²Neuroscience Center Zurich, University of Zurich and ETH Zurich

* These authors contributed equally

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我们描述了用小鼠大脑中表达荧光素酶 - 绿色荧光蛋白（GFP）的双报告载体转导的人神经祖细胞的实质内移植。移植后，使用荧光显微镜在脑切片 中鉴定的体内 生物发光和表达GFP的移植细胞反复测量荧光素酶信号。

Tags

神经科学，第179期，细胞移植，荧光素酶，体内成像，GFP，脑切片，实质内移植

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