Journal
/
Neurowissenschaften
/
在体内果蝇前运动神经末端的单分子跟踪
JoVE Journal
Neurowissenschaften
Author Produced
This content is Free Access.
JoVE Journal Neurowissenschaften
In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal
Please note that all translations are automatically generated. Click here for the English version.

在体内果蝇前运动神经末端的单分子跟踪

DOI:
10.3791/56952-v

•

06:45 min

•

January 14, 2018

•

, , , , ,
1Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute,The University of Queensland, 2VIB Centre for Brain and Disease Research, KU Leuven Department of Neurosciences,Leuven Institute for Neurodegenerative Disease (LIND), 3Queensland Brain Institute,The University of Queensland

Kapitel

  • 00:00Titel
  • 00:29Design of transgenic Drosophila
  • 00:44Dissection of third-instar Drosophila larva
  • 02:31Single particle tracking photo-activated localization microscopy
  • 04:31Data analysis
  • 05:13Representative results
  • 06:08Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

在这里, 我们说明如何单分子照片激活定位显微镜可以进行的运动神经终端的一个活的果蝇第三龄幼虫。

Tags

Single-molecule TrackingDrosophilaPresynaptic Motor Nerve TerminalIn VivoPhotoconvertible FluorophoreSchneider’s Insect MediumDissectionSylgard BaseTIRF MicroscopyNeuromuscular Junctions

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

在果蝇幼虫分部轴突在体内的突触小泡的可视化

膜片钳电容测量和Ca 2 +单神经末梢成像在视网膜切片

测量脊髓突触前抑制小鼠背根电位记录<em>在体内</em

为什么量化事项：表型的表征在<em>果蝇</em>幼虫神经肌肉接头

在毛囊披针形结局生活神经末端标记的光学监测<em>离体</em>鼠耳皮肤

通过计算机跟踪检测大鼠嗅觉行为<em>果蝇</em>在四象限嗅

体内基因转移到雪旺细胞在啮齿动物坐骨神经电穿孔

一个简单的一步解剖协议成人全安装准备<em>果蝇</em>脑

胚胎运动神经元轴突投影模式的可视化<em>果蝇</em

坐骨神经粉碎损伤大鼠模型中功能评估的3D运动学分析

Read Article