Journal
/
Immunologie und Infektion
/
Strongyloides Türlerinde Mikroenjeksiyon ile Transgenik ve Nakavt Üretimi
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Generating Transgenics and Knockouts in Strongyloides Species by Microinjection
Please note that all translations are automatically generated. Click here for the English version.

Strongyloides Türlerinde Mikroenjeksiyon ile Transgenik ve Nakavt Üretimi

2,390 Views

•

09:42 min

•

October 07, 2021

DOI:

10.3791/63023-v

DOI:
10.3791/63023-v

•

09:42 min
October 07, 2021

•

5 Views
,
1Department of Microbiology, Immunology, and Molecular Genetics,University of California, Los Angeles, 2Molecular Biology Institute,University of California, Los Angeles

Summary

Automatically generated

Parazitik nematodlar Strongyloides stercoralis ve Strongyloides ratti için fonksiyonel genomik araç seti transgenez, CRISPR / Cas9 aracılı mutagenez ve RNAi'yi içerir. Bu protokol, transgenleri ve CRISPR bileşenlerini S. stercoralis ve S . ratti'ye tanıtmak için intragonadal mikroenjeksiyonun nasıl kullanılacağını gösterecektir.

Explore More Videos

MicroinjectionTransgenicsKnockoutsStrongyloidesParasitesWormsBaermannFecal-charcoalNematode Growth MediumE. Coli HB101

Read Article

Cite this Article

Castelletto, M. L., Hallem, E. A. Generating Transgenics and Knockouts in Strongyloides Species by Microinjection. J. Vis. Exp. (176), e63023, doi:10.3791/63023 (2021).

Download .ris file

Copy

Share Video

.

Copy