/
/
Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices
This content is Free Access.
Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices
4,136 Views
•
10:48 min
•
September 07, 2021
DOI:
1Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute,KU Leuven – University of Leuven, 2VIB Bio Imaging Core,KU Leuven – University of Leuven, 3Department of Development and Regeneration, Stem Cell and Developmental Biology,KU Leuven – University of Leuven, 4Department of Neurology,University Hospitals Leuven
Summary
Automatically generated
We describe a method to generate human motor units in commercially available microfluidic devices by co-culturing human induced pluripotent stem cell-derived motor neurons with human primary mesoangioblast-derived myotubes resulting in the formation of functionally active neuromuscular junctions.
Read Article
Cite this Article
Stoklund Dittlau, K., Krasnow, E. N., Fumagalli, L., Vandoorne, T., Baatsen, P., Kerstens, A., Giacomazzi, G., Pavie, B., Rossaert, E., Beckers, J., Sampaolesi, M., Van Damme, P., Van Den Bosch, L. Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices. J. Vis. Exp. (175), e62959, doi:10.3791/62959 (2021).
Copy