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Single-Nucleus RNA 시퀀싱을 위한 성인 마우스 신장에서 핵 분리
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September 20, 2021
DOI:
1Department of Nephrology and Medical Intensive Care Medicine,Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements,Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB),Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
Summary
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여기에서 우리는 수질 신장 세포 유형의 표현을 개선하고 효소 조직 해리로 인한 유전자 발현 아티팩트를 방지하는 냉동 마우스 신장에서 고품질 핵을 분리하는 프로토콜을 제시합니다.
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Leiz, J., Hinze, C., Boltengagen, A., Braeuning, C., Kocks, C., Rajewsky, N., Schmidt-Ott, K. M. Nuclei Isolation from Adult Mouse Kidney for Single-Nucleus RNA-Sequencing. J. Vis. Exp. (175), e62901, doi:10.3791/62901 (2021).
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